FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n02_tsr05.35200000055d32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n02_tsr05.35200000055d32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14899088
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC37750.046.4186639
GATCGGG67300.035.522761
GGCGCCC14450.035.34280844-45
AAGGGGC39600.035.136279
TCGGGGG83400.034.80428338-39
GAGCGGC54050.034.7922679
GGGGCGC50450.033.47725342-43
GGGCGCC64600.032.21164742-43
AGGGGCG47300.030.32339910-11
GGGGCCC25250.030.00956544-45
AGAGCGG62150.029.8695348
GGGCGGG51300.029.30366512-13
GTAGGGG44950.029.16743718-19
ATCGGGA83100.028.8175012
CTCGGGG54800.028.4247236-37
GGGGGCG66600.027.10690940-41
TCGGGAG77250.026.3214233
GGGCCCG14850.026.2329944-45
AGGGTAG53350.025.50811828-29
CGGGGTG16950.024.38055614-15
Produced by FastQC (version 0.11.2)