FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n02_tsr02.35200000055d18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n02_tsr02.35200000055d18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16199176
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC86000.067.642179
GAGCGGC108150.060.9895749
GATCGGG130550.059.7060741
AAGGGGC72550.057.6658069
AGAGCGG120250.056.2643438
ATCGGGA151200.051.317782
TCGGGAG135150.050.6196943
CGGGAGA167150.046.4323654
GGCGCCC48500.041.97345744-45
TCGGGGG247550.041.2416938-39
GAGAGCG123500.041.170977
GGGCGCC192050.040.02591742-43
GGGGCGC162450.039.2034442-43
AGGGGCG119300.038.73443210-11
GTAGGGG117800.038.69187518-19
CTCGGGG152850.037.90458336-37
AGGGTAG122650.035.9203828-29
TAGGGGA128050.035.52051518-19
GAGAGGG185950.035.4374057
CGGGTAG70050.035.2606114-15
Produced by FastQC (version 0.11.2)