FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n01_tsr35.35100000055e84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n01_tsr35.35100000055e84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9376903
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC742220.7915406611329988TruSeq Adapter, Index 11 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT176850.021.9030248-49
TGAAAAA173550.021.59318262-63
ATGCCGT182100.021.09008846-47
TGCCGTC180900.020.97941448-49
CTGCTTG188700.020.74317256-57
TATGCCG185850.020.33227246-47
GAAAAAA188950.020.09721662-63
TTGAAAA192800.019.91856860-61
GCTTGAA205450.019.08669958-59
GTATGCC206250.018.747344-45
CGTATGC210200.018.01083844-45
CTTGAAA212450.017.8079660-61
TGCTTGA219450.017.67422958-59
CCGTCTT220350.017.51459350-51
CGCGGAT14300.017.2772731
TCGTATG229000.016.8226242-43
CGGGGGT7450.015.9437921
TTCTGCT249800.015.91669554-55
CTCGTAT255300.014.95007442-43
CTTCTGC263450.014.803514554-55
Produced by FastQC (version 0.11.2)