FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n01_tsr32.35100000055e6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n01_tsr32.35100000055e6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7213588
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC472120.6544870596990014TruSeq Adapter, Index 10 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT11550.020.1580181
TGAAAAA132400.017.54227862-63
CTGCTTG140650.017.20785756-57
CCGGGGT4700.017.1863961
GCCGTCT143550.016.54407548-49
GCTTGAA147300.016.35036858-59
TTGAAAA145900.016.24538460-61
CGCGCCT8200.016.2247471
TGCCGTC147300.015.9455448-49
GAAAAAA148800.015.84825662-63
ATGCCGT149700.015.81851646-47
TGCTTGA154850.015.44580758-59
CTTGAAA156200.015.12853160-61
TATGCCG156550.014.89878446-47
CGCGTAT7300.014.3196891
CGCGGGT7100.014.0538311
CGCGGCT11950.013.9166231
CCGTCTT173900.013.90277250-51
CGGGCAT11250.013.5154721
GTATGCC176150.013.42978544-45
Produced by FastQC (version 0.11.2)