FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n01_tsr29.35100000055e41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n01_tsr29.35100000055e41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10663706
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC396290.3716250241707714TruSeq Adapter, Index 9 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT19850.022.2618331
CGCGCCT17400.016.3847751
CGCGCGT19300.016.2489411
GGAGCCC16950.015.1321138
CCGACTG31000.015.0180392
CGACTGT35000.014.929423
CCCGACT40450.014.4485761
CGGGGGA13200.014.3987411
GCCGTCT156100.013.47967448-49
TGAAAAA157500.013.14835162-63
ATGCCGT158650.013.14452146-47
CTGCTTG166800.013.11398256-57
CGCGGCT27050.012.8231311
TGCCGTC164000.012.56968748-49
TATGCCG162200.012.56396746-47
GACTGTC40050.012.2149564
CCGGAAC56500.012.1943321
CCGACAT36350.012.0260191
CCCGGGT12250.012.0244181
GCTTGAA183650.011.923614558-59
Produced by FastQC (version 0.11.2)