FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n01_tsr26.35100000055e27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n01_tsr26.35100000055e27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13852021
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1284380.9272148807744373TruSeq Adapter, Index 8 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA287050.023.44665762-63
GCCGTCT296500.023.36480348-49
TGCCGTC300950.022.6878848-49
ATGCCGT304200.022.63510546-47
CTGCTTG311450.022.4506556-57
TATGCCG310500.021.86986446-47
GAAAAAA315600.021.52878662-63
TTGAAAA322350.021.32892660-61
GCTTGAA327750.021.23990258-59
GTATGCC340000.020.30066344-45
TGCTTGA344300.020.08786458-59
CTTGAAA344100.020.00836460-61
CGTATGC344750.019.77982344-45
CCGTCTT354600.019.65031250-51
CGCGGAT23350.018.9246461
TCGTATG376000.018.40751842-43
TTCTGCT402750.017.72100454-55
CTTCTGC418450.016.8517954-55
CGTCTTC424300.016.59585850-51
CCGGGGT10050.016.547551
Produced by FastQC (version 0.11.2)