FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n01_tsr23.35100000055dff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n01_tsr23.35100000055dff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11767977
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC831380.7064765677227276TruSeq Adapter, Index 3 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT193900.022.6837248-49
ATGCCGT196000.022.3094846-47
TATGCCG196750.022.06750146-47
TGCCGTC198350.022.03112848-49
CTGCTTG217150.020.45290856-57
TGAAAAA214750.020.17085662-63
CGTATGC215900.020.09914844-45
GTATGCC218250.020.04596944-45
CGCGGAT21350.019.1400951
GCTTGAA239900.018.39453158-59
TCGTATG238400.018.33172442-43
TTGAAAA239450.018.32886160-61
GAAAAAA236950.018.30107962-63
CCGTCTT244100.018.18405750-51
TGCTTGA246850.018.01133758-59
CGCGCCT16000.017.8186511
CTTGAAA252400.017.62368860-61
CTCGTAT269600.016.22786942-43
TTCTGCT300500.015.05649154-55
CCGGGGT8600.014.9179391
Produced by FastQC (version 0.11.2)