FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n01_tsr20.35100000055dd5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n01_tsr20.35100000055dd5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13051086
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1569981.202949700890792TruSeq Adapter, Index 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT301650.028.40618748-49
ATGCCGT301600.028.20848746-47
TGCCGTC302500.027.96522348-49
TATGCCG304200.027.70973246-47
CTGCTTG332400.025.95187256-57
GTATGCC328800.025.93272644-45
CGTATGC328100.025.69125444-45
TGAAAAA323650.025.49078262-63
GCTTGAA348050.024.63482358-59
TCGTATG353100.024.12115542-43
CCGTCTT358550.023.86556650-51
TGCTTGA357000.023.81098658-59
TTGAAAA356800.023.63640460-61
GAAAAAA354850.023.42352162-63
CTTGAAA365000.023.17696860-61
CTCGTAT371050.021.55882842-43
TTCTGCT414650.020.98735254-55
CGTCTTC422450.020.60982950-51
CTTCTGC422950.020.36210354-55
TCTGCTT435800.019.77805756-57
Produced by FastQC (version 0.11.2)