FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n01_tsr17.35100000055dbb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n01_tsr17.35100000055dbb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9290528
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC949061.0215350516138588TruSeq Adapter, Index 12 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT189400.026.63639546-47
GCCGTCT189750.026.62180548-49
TATGCCG189900.026.17852246-47
TGCCGTC195900.025.36173848-49
GTATGCC204550.024.65195844-45
CTGCTTG209350.024.471356-57
CGTATGC206400.024.13179644-45
TGAAAAA207400.023.77072162-63
GCTTGAA219850.022.97845858-59
TCGTATG222950.022.67070442-43
TGCTTGA225100.022.4108858-59
CCGTCTT224000.022.39164450-51
TTGAAAA229100.021.98642260-61
GAAAAAA229150.021.78394962-63
CTTGAAA235750.021.1143960-61
CTCGTAT243750.020.35610642-43
TTCTGCT271300.019.00596254-55
CTTCTGC271900.018.77184954-55
CGTCTTC283350.018.1876550-51
TCTCGTA283500.017.83658840-41
Produced by FastQC (version 0.11.2)