FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n01_tsr14.35100000055d92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n01_tsr14.35100000055d92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12388486
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC989940.7990806947677062TruSeq Adapter, Index 7 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT217550.023.51670846-47
GCCGTCT218850.023.43967248-49
TATGCCG218550.023.22435646-47
TGCCGTC220000.023.21999248-49
CGTATGC234450.021.65944944-45
GTATGCC237800.021.49415244-45
CTGCTTG245300.021.2334956-57
TGAAAAA248200.020.42827462-63
TCGTATG257850.019.85043742-43
GCTTGAA265650.019.57110658-59
TTGAAAA269850.019.12447760-61
TGCTTGA272050.019.10196358-59
CCGTCTT277800.018.77399650-51
GAAAAAA274700.018.69100462-63
CTTGAAA279550.018.5458460-61
CGCGGAT23700.017.6426871
CTCGTAT288400.017.63239742-43
TTCTGCT329950.016.12430854-55
CGTCTTC332000.015.82354250-51
CTTCTGC341000.015.42071154-55
Produced by FastQC (version 0.11.2)