FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n01_tsr11.35100000055d78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n01_tsr11.35100000055d78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12060480
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1090210.9039524131709517TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA226850.024.42390662-63
GCCGTCT232050.024.26574748-49
ATGCCGT239050.023.5074146-47
TATGCCG237300.023.45056546-47
TGCCGTC242750.022.96135548-49
CTGCTTG251650.022.77432456-57
TTGAAAA263000.021.57326160-61
GTATGCC262750.021.44128244-45
CCGTCTT264000.021.30271550-51
GAAAAAA264150.021.01102662-63
TGCTTGA276450.020.4820758-59
CGTATGC274700.020.30968344-45
CTTGAAA287550.019.60752360-61
TCGTATG300550.018.82364842-43
GCTTGAA304250.018.81354758-59
TTCTGCT318700.018.10220354-55
CTCGTAT320450.017.46199242-43
CGTCTTC331000.017.43554350-51
CTTCTGC337400.017.03555554-55
TCTGCTT355950.016.24114656-57
Produced by FastQC (version 0.11.2)