FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n01_tsr08.35100000055d5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n01_tsr08.35100000055d5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10139238
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC842560.8309894688338513TruSeq Adapter, Index 5 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA186050.023.74166362-63
GCCGTCT205700.021.76300448-49
CTGCTTG208250.021.70409256-57
TGCCGTC210200.021.28579748-49
TATGCCG213200.020.88834446-47
ATGCCGT214100.020.8116346-47
GAAAAAA213200.020.62916462-63
TTGAAAA222400.020.0218460-61
TGCTTGA230150.019.6697958-59
CCGTCTT229100.019.60230450-51
GTATGCC235450.018.9043144-45
CTTGAAA244400.018.53049360-61
CGTATGC247950.018.03749344-45
GCTTGAA254700.017.94171358-59
TTCTGCT268800.017.080154-55
CGGGGCG1952.2721842E-417.0565221
CTTCTGC279950.016.45921154-55
TCGTATG271050.016.44768542-43
TCTGCTT290350.015.86148356-57
CGTCTTC287900.015.78850350-51
Produced by FastQC (version 0.11.2)