FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n01_tsr05.35100000055d35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n01_tsr05.35100000055d35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14899088
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1200930.8060426248908659TruSeq Adapter, Index 4 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA272550.022.9684862-63
CTGCTTG310300.020.85016356-57
GCCGTCT316000.020.35964848-49
GAAAAAA314000.020.14826462-63
TTGAAAA323800.019.86217360-61
TGCCGTC324950.019.49192848-49
ATGCCGT331150.019.4159146-47
TGCTTGA337400.019.14021158-59
TATGCCG332500.019.00134346-47
CCGTCTT348750.018.20973450-51
CTTGAAA358500.017.8734260-61
GTATGCC365450.017.62609144-45
GCTTGAA373700.017.58606558-59
TTCTGCT397700.016.5786654-55
CGTATGC383800.016.52963344-45
CTTCTGC415650.015.52556454-55
TCGTATG420350.015.36922942-43
CGTCTTC433150.015.23176850-51
TCTGCTT437750.014.92617456-57
CTCGTAT444400.014.05111542-43
Produced by FastQC (version 0.11.2)