FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l08n01_tsr02.35100000055d1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l08n01_tsr02.35100000055d1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16199176
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC4328752.6722038207375487TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA611500.035.27465462-63
CTGCTTG657650.033.70239656-57
TTGAAAA660900.033.05624860-61
GAAAAAA662850.032.52765362-63
GCCGTCT680350.032.4496848-49
TGCTTGA687700.032.04312558-59
TGCCGTC693550.031.70880948-49
CTTGAAA696650.031.42808260-61
ATGCCGT705750.031.25168446-47
CCGTCTT712150.030.94785550-51
TATGCCG713700.030.81038546-47
GCTTGAA728000.030.39980958-59
GTATGCC752900.029.36396644-45
TTCTGCT759100.029.34851354-55
CTTCTGC773100.028.72487654-55
CGTATGC776600.028.36997844-45
CGTCTTC792700.028.09371250-51
TCTGCTT796650.027.91738556-57
TCGTATG823800.026.85118542-43
GTCTTCT828250.026.78323252-53
Produced by FastQC (version 0.11.2)