FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n02_tsr31.35200000055e5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n02_tsr31.35200000055e5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13319941
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC8700.033.58556444-45
GGGCGCC30100.030.46473542-43
GGGGCGC29050.027.06814242-43
TCGGGGG48950.026.7878438-39
GGGGCCC13100.025.75039544-45
GAGCGGC36850.024.6156649
GAGGGGC24550.024.1809989
GGGCGGG27600.023.92378612-13
AAGGGGC31250.023.0998559
GGGGGCG35900.022.96201540-41
GGGCCCG8300.022.61079444-45
AGGGGCG27750.020.88286210-11
CTCGGGG35750.020.5229436-37
GCGCCCG11000.018.78855544-45
GATCGGG52250.018.5521621
GTAGGGG29250.018.43465218-19
GGGGCCG19300.018.33984844-45
CCGGGGG26250.017.55592538-39
AGAGCGG54100.017.2911038
GGGGCGG32450.016.97995410-11
Produced by FastQC (version 0.11.2)