FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n02_tsr28.35200000055e31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n02_tsr28.35200000055e31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14443506
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC8850.029.52653144-45
GGGCGCC25500.028.7865542-43
GGGGCGC24850.024.37727442-43
TCGGGGG42700.024.3665638-39
GGGGCCC13050.023.66442144-45
GGGCCCG7800.022.23265344-45
GAGGGGC24850.021.5961999
GGGGGCG31650.020.56592240-41
GGGCGGG27350.019.1929112-13
GCGCCCG11250.019.0043544-45
GAGCGGC33500.018.997019
GTAGGGG25900.018.52618618-19
CTCGGGG31550.017.76133336-37
AGGGGCG25900.017.60663810-11
AAGGGGC30000.017.2556179
GGGGGCC22900.016.07931542-43
GGGGGGC39400.015.13382940-41
GGGCCCC8100.014.6638544-45
CGGGGGG48300.014.01669838-39
GGGGCGG31250.013.90836510-11
Produced by FastQC (version 0.11.2)