FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n02_tsr25.35200000055e17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n02_tsr25.35200000055e17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13073483
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCC25050.032.62326442-43
GGGGCGC22850.028.48662842-43
GGCGCCC7100.027.43624744-45
GAGGGGC23450.026.5303089
TCGGGGG40800.025.61789538-39
GGGGCCC11300.023.75577744-45
GGGGGCG29700.023.19609840-41
GAGCGGC32300.023.0839989
GGGCGGG25050.022.37730812-13
GTAGGGG23650.021.69523618-19
GGGCCCG7350.021.65492444-45
AGGGGCG24300.020.9157210-11
CTCGGGG30650.020.52903636-37
AAGGGGC28400.018.3945089
GATCGGG53650.017.9787561
CGGGTAG16350.017.5790114-15
TAGGGGA30400.017.19059418-19
GGGGCGG26950.016.83216310-11
AGAGCGG49700.016.4326578
GGCGGGG34650.016.24608212-13
Produced by FastQC (version 0.11.2)