FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n02_tsr22.35200000055e0a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n02_tsr22.35200000055e0a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12014961
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCC13500.036.95305644-45
GAGCGGC35500.036.7901769
GGGCGCC40900.036.6507342-43
GAGGGGC31250.033.7388659
GGGGCGC38550.033.15389342-43
TCGGGGG63900.032.45420538-39
AAGGGGC31450.031.5611749
GATCGGG55000.030.6701351
GGGGCCC16650.029.10588344-45
GTAGGGG30750.028.04131118-19
GCGCCCG13000.027.5929744-45
CGGGTAG18200.027.53840814-15
GGGGGCG52000.027.13675540-41
GGGCGGG38150.026.7724512-13
AGGGGCG34450.026.68113310-11
AGAGCGG50150.026.6062328
CTCGGGG43500.024.83641636-37
TCGGGAG56550.024.5261973
GGGCCCG9400.024.51369344-45
ATCGGGA68550.024.3917142