FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n02_tsr19.35200000055def.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n02_tsr19.35200000055def.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13552382
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCC42550.036.51357742-43
GAGGGGC30750.035.0604069
GAGCGGC39300.034.804569
GGCGCCC13600.033.71222744-45
TCGGGGG66100.032.8109738-39
GGGGCGC37900.032.4061642-43
AAGGGGC34050.030.267669
GATCGGG62700.029.2530331
AGGGGCG34150.027.54099810-11
GGGGGCG51550.026.5902140-41
GTAGGGG33200.026.04326418-19
GGGGCCC19950.025.95868144-45
CGGGTAG21550.025.35132614-15
AGAGCGG57000.024.9914388
GGGCGGG40350.024.42898412-13
CTCGGGG46300.024.3087136-37
ATCGGGA75750.024.0174962
TCGGGAG62850.023.2014873
GGGCCCG11350.022.81390644-45
GCGCCCG15500.022.37639844-45