FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n02_tsr16.35200000055dc5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n02_tsr16.35200000055dc5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12495173
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCC39000.038.06993542-43
GAGGGGC31200.037.4456679
GGCGCCC12850.035.67896344-45
GGGGCGC36550.034.05733542-43
TCGGGGG63350.033.93472738-39
GAGCGGC38150.032.242329
AAGGGGC33150.031.6613799
GATCGGG55750.030.5974061
GGGGCCC19400.028.89803544-45
AGGGGCG34400.028.65278210-11
GTAGGGG34350.027.79941218-19
GGGCGGG39850.026.70229712-13
GGGGGCG51350.026.46187440-41
CGGGTAG21700.025.61396414-15
CTCGGGG45350.025.55097836-37
ATCGGGA68550.024.9458262
GCGCCCG13600.024.80318644-45
GGGCCCG12800.024.49751544-45
AGAGCGG52750.023.7645458
TCGGGAG61050.023.5740243