FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n02_tsr13.35200000055dac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n02_tsr13.35200000055dac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11265225
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC36150.048.6071059
GAGCGGC43750.047.0019879
GATCGGG65550.043.130461
AAGGGGC36800.041.94139
GGGCGCC56450.039.8112842-43
GGCGCCC17250.039.52428444-45
AGAGCGG54550.038.2989738
TCGGGGG84450.037.8615438-39
GGGGCGC50300.036.93326642-43
TCGGGAG63250.036.19553
ATCGGGA77900.036.0984842
GTAGGGG43350.034.3575118-19
AGGGGCG44100.033.8212810-11
GCGCCCG15750.033.33365644-45
CGGGAGA80150.031.7577884
GGGCGGG54000.031.58147612-13
CGGGTAG28250.031.44733614-15
CTCGGGG53950.029.79541836-37
TAGGGGA50450.029.28683318-19
GGGGCCC28550.029.03959344-45