FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n02_tsr10.35200000055d82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n02_tsr10.35200000055d82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15352964
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC40200.044.3024839
GAGCGGC56150.038.9072849
AAGGGGC42500.038.887789
GATCGGG71950.038.503341
GGCGCCC22100.036.76172344-45
TCGGGGG98600.036.18614238-39
GGGGCGC58350.035.6238842-43
AGAGCGG66950.033.3354578
GTAGGGG47950.033.1407818-19
GGGCGCC76650.032.82145342-43
AGGGGCG49750.031.9847710-11
ATCGGGA90400.030.6358952
TCGGGAG76700.030.2838673
GGGCGGG59200.029.16738912-13
CTCGGGG67650.028.60576836-37
GGGCCCG18500.028.50645644-45
GCGCCCG20550.028.20590444-45
GGGGGCG81000.027.86183740-41
CGGGTAG32750.027.56021714-15
GGGGCCC33050.027.38517444-45