FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n02_tsr04.35200000055d4f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n02_tsr04.35200000055d4f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17925191
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC48700.050.2228139
AAGGGGC52300.045.4944959
GATCGGG91850.042.4716341
GAGCGGC72300.040.8577889
GGCGCCC28000.038.26381744-45
TCGGGGG125050.037.55527538-39
AGAGCGG81050.037.201458
GGGGCGC76400.036.10082642-43
AGGGGCG62650.035.1425510-11
GGGCGCC96800.034.922742-43
GTAGGGG60800.033.7940718-19
ATCGGGA116000.033.4152452
TCGGGAG96850.032.2705273
GGGCGGG75350.032.02684812-13
CTCGGGG81450.030.28892936-37
GCGCCCG26800.029.51747744-45
GGGGCCC40700.029.35914444-45
CGGGTAG40550.029.17148614-15
AGGGTAG76750.029.02946928-29
GGGCCCG22500.028.82371344-45
Produced by FastQC (version 0.11.2)