FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n01_tsr34.35100000055e77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n01_tsr34.35100000055e77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13964138
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC824850.5906916703343952TruSeq Adapter, Index 11 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT22750.022.9702471
CCGGGGT9500.018.002521
TGAAAAA234600.017.88695762-63
GCCGTCT255550.016.97971248-49
CTGCTTG261900.016.8608156-57
ATGCCGT259700.016.627246-47
TGCCGTC259350.016.54777148-49
GAAAAAA254350.016.53540462-63
TTGAAAA264300.016.048860-61
TATGCCG269950.015.67031646-47
GCTTGAA283300.015.42783458-59
CGCGCCT14850.015.3556841
CTTGAAA286950.014.88960660-61
TGCTTGA300750.014.55638158-59
CGGGGGA17150.014.4043471
GTATGCC301800.014.33119644-45
CCGTCTT312900.013.90515550-51
GCGGGAT24200.013.7415911
CGTATGC309050.013.741384544-45
TTCTGCT348250.012.96660454-55
Produced by FastQC (version 0.11.2)