FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n01_tsr31.35100000055e5e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n01_tsr31.35100000055e5e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13319941
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC610080.4580200467854925TruSeq Adapter, Index 10 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT23350.020.346211
CGCGCCT17650.018.303521
CCGGGGT9650.017.2310261
TGAAAAA203500.015.28772762-63
CGCGGCT24100.013.996251
GAAAAAA226800.013.79046162-63
CTGCTTG236000.013.74865956-57
CCCGGGT14900.013.7104771
TTGAAAA235000.013.4815460-61
CCCGACT28700.013.242761
GCTTGAA251900.012.880815558-59
CGCGGGT10550.012.6088641
CTTGAAA255700.012.60377560-61
GCCGTCT254350.012.51283148-49
CGCGCGT14650.012.3229981
CGGGGGT10200.012.1099851
TGCTTGA271050.012.05840558-59
TGCCGTC267700.011.84446448-49
GCCGGGT19650.011.6051051
ATGCCGT276650.011.4700846-47
Produced by FastQC (version 0.11.2)