FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n01_tsr28.35100000055e34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n01_tsr28.35100000055e34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14443506
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC462940.320517746868385TruSeq Adapter, Index 9 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT10750.022.0968191
CGCGGAT22500.020.4812951
CGCGCCT16450.015.8841881
CGGGGGA20600.014.9904381
CCCGGGT16350.014.8190581
CGGGGGT10500.013.5737581
CGCGGCT30200.013.0568781
CGCGCGT16250.012.8637481
CCCGACT29950.012.0554931
CGGGGCG4405.806396E-611.8770391
TGAAAAA198800.011.71890662-63
CCGGGTT51950.011.7055711
GCGGGTT33450.011.6462451
GCCGTCT219700.011.09178348-49
CTGCTTG228450.010.99061656-57
CGCGGTT27000.010.9092811
ATGCCGT223050.010.75469146-47
TGCCGTC220950.010.63129948-49
GAAAAAA227500.010.56412662-63
CTGGGAT56350.010.5386321
Produced by FastQC (version 0.11.2)