FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n01_tsr25.35100000055e1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n01_tsr25.35100000055e1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13073483
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC489650.37453676269743874TruSeq Adapter, Index 8 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT24100.022.0786631
CCGGGGT9850.018.328171
CGGGGGT11400.016.2529261
CGCGCCT17350.013.9650491
CGCGGCT24800.013.4096721
CGGGGTG7650.013.0415621
TGAAAAA193450.012.98815362-63
CGGATGG40400.012.9336643
GCCGTCT205700.012.63140648-49
GCGGATG43550.012.5437752
CGCGGTA18100.012.3364731
ATGCCGT210900.012.23078446-47
GAAAAAA211400.012.17740562-63
TGCCGTC208400.012.11446548-49
CTGCTTG220400.012.113090556-57
GCCGGGT16750.011.9125911
CCGGGTT50100.011.8534311
CGGGCGT8450.011.8068581
TTGAAAA226950.011.59451360-61
CGGGGGA18500.011.5561331
Produced by FastQC (version 0.11.2)