FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n01_tsr22.35100000055e0d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n01_tsr22.35100000055e0d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12014961
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC883490.7353249003471588TruSeq Adapter, Index 3 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT216250.021.92183148-49
ATGCCGT216750.021.72926746-47
TGCCGTC217900.021.4724448-49
TATGCCG222850.020.90000546-47
CTGCTTG234750.020.51068756-57
TGAAAAA227450.020.1718962-63
GTATGCC246350.019.19545444-45
CGCGGAT20800.019.1863561
GAAAAAA241950.019.15929862-63
CGTATGC245050.019.00651644-45
GCTTGAA261050.018.35330658-59
TTGAAAA255850.018.34242660-61
TGCTTGA269100.017.74248558-59
TCGTATG269300.017.57723642-43
CTTGAAA271400.017.37024360-61
CCGTCTT274700.017.17890250-51
TTCTGCT313800.015.68443654-55
CTCGTAT306200.015.45125742-43
CTTCTGC325650.014.93863654-55
TCTGCTT328300.014.90487856-57
Produced by FastQC (version 0.11.2)