FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n01_tsr19.35100000055de2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n01_tsr19.35100000055de2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13552382
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC839250.6192638312585935TruSeq Adapter, Index 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT220500.021.82255748-49
ATGCCGT219650.021.79984346-47
TGCCGTC221400.021.4334848-49
TATGCCG222700.021.1386646-47
GTATGCC246400.019.56795144-45
CTGCTTG253050.019.4487256-57
CGTATGC245150.019.27049644-45
TGAAAAA245500.019.04696362-63
CGCGGAT24850.018.544361
TCGTATG268750.017.98481442-43
GAAAAAA265550.017.84136862-63
TTGAAAA273150.017.54550760-61
GCTTGAA279600.017.37255958-59
TGCTTGA287450.016.79070958-59
CCGTCTT288350.016.74450150-51
CTTGAAA292400.016.3254360-61
CCGGGGT10200.016.3017561
CTCGTAT295300.015.07287842-43
TTCTGCT343600.014.42017154-55
CGTCTTC348150.014.33907450-51
Produced by FastQC (version 0.11.2)