FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n01_tsr16.35100000055dc8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n01_tsr16.35100000055dc8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12495173
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC905640.7247918856345567TruSeq Adapter, Index 12 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT236900.020.2318748-49
ATGCCGT242600.019.69828446-47
TGCCGTC239000.019.46778348-49
TGAAAAA237000.019.4093862-63
CTGCTTG257400.018.95428856-57
TATGCCG248800.018.63462446-47
GAAAAAA258550.018.20495262-63
GCTTGAA269800.017.96869958-59
TTGAAAA270050.017.73809260-61
GTATGCC276450.017.35491844-45
CGTATGC276100.016.80055444-45
TGCTTGA283800.016.7809958-59
CTTGAAA284000.016.55739660-61
CGCGGAT23350.016.4800171
CCGTCTT287250.016.3872250-51
TCGTATG302800.015.82898542-43
CCGGGGT9200.015.4914791
TTCTGCT339850.014.58648854-55
CGTCTTC346050.014.23415150-51
CTCGTAT336550.013.797008542-43
Produced by FastQC (version 0.11.2)