FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n01_tsr13.35100000055daf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n01_tsr13.35100000055daf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11265225
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1436981.2755892580929364TruSeq Adapter, Index 7 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT251400.030.00698346-47
TGCCGTC252950.029.9057648-49
GCCGTCT254800.029.67930648-49
TATGCCG254050.029.64723246-47
GTATGCC273650.027.6015844-45
CGTATGC272550.027.59097344-45
CTGCTTG291150.026.45697456-57
TCGTATG291150.025.94254942-43
TGAAAAA287050.025.8119262-63
GCTTGAA305850.024.90579858-59
CCGTCTT308150.024.73246650-51
TGCTTGA313900.024.37302258-59
TTGAAAA308500.024.31850860-61
GAAAAAA305400.024.1754762-63
CTCGTAT319800.023.62585342-43
CTTGAAA324250.023.35699860-61
TTCTGCT359200.021.56375754-55
CGTCTTC357600.021.39872650-51
TCTCGTA357100.020.99071540-41
CTTCTGC372050.020.74237354-55
Produced by FastQC (version 0.11.2)