FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n01_tsr10.35100000055d85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n01_tsr10.35100000055d85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15352964
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1560431.0163705197250512TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG313450.026.3081246-47
GCCGTCT317750.026.07233248-49
TGAAAAA310050.025.98116762-63
ATGCCGT318750.025.93029446-47
TGCCGTC320100.025.85124448-49
GTATGCC341450.024.23404544-45
CTGCTTG350600.023.98461256-57
CGTATGC350050.023.56403244-45
CCGTCTT354050.023.45972350-51
GAAAAAA346600.023.24137562-63
TTGAAAA352250.023.15295660-61
TGCTTGA373600.022.43174458-59
TCGTATG383150.021.6027442-43
CTTGAAA390650.021.13241460-61
GCTTGAA410450.020.46413458-59
CTCGTAT405850.020.45882642-43
TTCTGCT440750.019.28371854-55
CGTCTTC443300.019.01516550-51
CTTCTGC453600.018.68506254-55
TATCTCG449250.018.26438738-39
Produced by FastQC (version 0.11.2)