FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n01_tsr07.35100000055d6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n01_tsr07.35100000055d6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14393528
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1328830.923213544309637TruSeq Adapter, Index 5 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA277850.024.35016862-63
CTGCTTG312650.022.3742656-57
GCCGTCT309650.022.32788748-49
TGCCGTC319650.021.56993548-49
TTGAAAA323000.021.32234860-61
ATGCCGT322750.021.29763646-47
TATGCCG324050.021.21219846-47
GAAAAAA318300.021.21095362-63
TGCTTGA336450.020.85507658-59
CCGTCTT343150.020.2230750-51
CTTGAAA358250.019.489560-61
GTATGCC359100.019.2343144-45
GCTTGAA370550.019.07049458-59
CGTATGC376000.018.29398544-45
TTCTGCT392700.018.04328754-55
CTTCTGC410000.017.17187154-55
TCTGCTT426050.016.68660456-57
CGTCTTC421550.016.67042750-51
TCGTATG415700.016.66115642-43
GTCTTCT455600.015.57203552-53
Produced by FastQC (version 0.11.2)