FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n01_tsr04.35100000055d42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n01_tsr04.35100000055d42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17925191
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1976051.1023871377437484TruSeq Adapter, Index 4 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA385650.026.75013462-63
GCCGTCT405150.026.47946248-49
ATGCCGT411550.026.08042346-47
TATGCCG406850.025.8679746-47
TGCCGTC411850.025.66808548-49
CTGCTTG428000.025.35122956-57
GTATGCC441000.024.34395244-45
GAAAAAA433500.024.12613362-63
TTGAAAA442100.024.1143860-61
CCGTCTT450450.023.58911150-51
CGTATGC454500.023.14531344-45
TGCTTGA460000.023.128158-59
TCGTATG492600.021.8324942-43
GCTTGAA500850.021.6353758-59
CTTGAAA492900.021.5182560-61
TTCTGCT525500.020.95498754-55
CTCGTAT505550.020.31481442-43
CGTCTTC549150.019.91594150-51
CTTCTGC547100.019.69784754-55
TCTGCTT579450.018.71701656-57
Produced by FastQC (version 0.11.2)