FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l07n01_tsr01.35100000055d28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l07n01_tsr01.35100000055d28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19093170
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC8371644.384625496970907TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA1092550.038.99958462-63
CTGCTTG1171250.037.8244756-57
TTGAAAA1146050.037.659560-61
GCCGTCT1184150.037.2328248-49
GAAAAAA1151200.036.89509262-63
TGCTTGA1195950.036.83473658-59
TGCCGTC1198150.036.75415448-49
ATGCCGT1202550.036.61480746-47
TATGCCG1207050.036.46846446-47
CTTGAAA1201250.036.1563360-61
CCGTCTT1222300.036.04973250-51
GCTTGAA1229800.035.65476258-59
GTATGCC1251550.035.2568744-45
CGTATGC1277300.034.4828844-45
TTCTGCT1289950.034.43421654-55
CTTCTGC1313250.033.7617854-55
CGTCTTC1322050.033.505850-51
TCGTATG1323700.033.3764242-43
TCTGCTT1331800.033.35564856-57
GTCTTCT1360550.032.55418452-53
Produced by FastQC (version 0.11.2)