FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l05n02_drought_1h_rep2_shoot.35200000055c90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l05n02_drought_1h_rep2_shoot.35200000055c90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27406698
Sequences flagged as poor quality0
Sequence length101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGC1004.9651884E-528.4932569
TCGGTGG2700.019.35603738-39
GGTAGGG757.719245E-419.00296416-17
CTGGGGG901.2326095E-418.47621738-39
TCGGGGG2153.6379788E-1217.6782438-39
GAGTGTA2051.737335E-713.90782226-27
CTCGGGG1402.2900257E-413.56852736-37
GGGGGCC3700.013.48372842-43
GGCGGGT1453.07547E-413.10387512-13
GGGGGGC4950.012.95838842-43
TCCGGGG1751.0943991E-412.21167436-37
CGGGGGG6400.011.87756838-39
GCCGGGT1400.003509814211.87692846-47
AGGGCGG1607.055495E-411.87414110-11
AAGAGTG2451.5086262E-611.63735924-25
GGGCCGG2907.9657184E-811.46872944-45
GTAGGGA3152.0610969E-811.31150418-19
CTCGGTG2951.01630576E-711.26877636-37
GTGGTCG3001.2732562E-711.08662140-41
GGGGAAT2003.778728E-410.68639530-31
Produced by FastQC (version 0.11.2)