FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l05n02_drought_1h_rep1_shoot.35200000055bfe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l05n02_drought_1h_rep1_shoot.35200000055bfe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26858150
Sequences flagged as poor quality0
Sequence length101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGGG1154.285031E-516.5180236-37
TCGGGGG1453.0704465E-413.10640938-39
TAGGGGA1607.042248E-411.87679818-19
GGGGGCC1952.978303E-410.96498642-43
CGGGGGG3458.6915134E-79.63985938-39
TCGGTGG2200.0098673368.63831538-39
TTCGGGG2200.0099037788.6344236-37
GGTAAGT2900.0012128458.18853732-33
GGGGAAG3954.9379025E-57.81911120-21
GGGGGGA3200.0031842667.422998418-19
GGCGGGG3200.00318859177.42196812-13
Produced by FastQC (version 0.11.2)