FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l05n02_drought_1h_rep1_root.35200000055c26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l05n02_drought_1h_rep1_root.35200000055c26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19935346
Sequences flagged as poor quality0
Sequence length101
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGTC857.951361E-519.56236892-93
TCGGTGG6300.09.80343794-95
AAAGAGG5154.638423E-109.2250494-95
CGGTGGT6302.7830538E-108.29521694-95
GAGTGTA15100.08.02303694-95
GTGTAGA14300.07.807416494-95
CTCGGTG7002.8921932E-107.80498592-93
GTAGATC12800.07.794439394-95
TGTAGAT13550.07.538322494-95
GTGGTCG5651.6074991E-67.14736694-95
AGTGTAG14300.07.14290292-93
AGAGTGT19850.07.060525494-95
AAGAGTG18900.06.9126894-95
TCGTGTA40200.06.73634694-95
CGTGTAG37650.06.687848694-95
AAAGAGT22200.06.420081692-93
TGTAGGG30550.06.375998594-95
GTAGGGA28900.06.00026794-95
GTCGTGT44000.05.992607694-95
GTGTAGG32800.05.721353594-95
Produced by FastQC (version 0.11.2)