FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l05n01_drought_1h_rep2_shoot.35100000055c93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l05n01_drought_1h_rep2_shoot.35100000055c93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27406698
Sequences flagged as poor quality0
Sequence length101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCC6700.034.02602820-21
AGAGCAC8150.033.7996988
ACACGTC7200.033.31277512-13
GCACACG7250.032.75481810-11
CAGTCAC7000.031.89418826-27
GCTTGAA8250.027.63252660-61
TATCTCG8650.026.91008840-41
AGCACAC7600.026.8718819
GTCACAG8450.026.42095628-29
TCTCGTA9100.026.36308342-43
TCCAGTC8900.025.88579624-25
ACTCCAG8750.025.78272222-23
GAGCACA12300.022.3957759
CACAGTT10400.021.92341230-31
CTGCTTG11100.020.5396258-59
CCAGTCA6500.020.09699226-27
TGAACTC6950.019.81791520-21
TCACAGT7350.019.71118730-31
CACACGT7150.019.5960212-13
CACGTCT7200.019.4615214-15
Produced by FastQC (version 0.11.2)