FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l05n01_drought_1h_rep1_shoot.35100000055bf1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l05n01_drought_1h_rep1_shoot.35100000055bf1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26858150
Sequences flagged as poor quality0
Sequence length101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCC3950.027.05381620-21
AGAGCAC5950.025.5431798
GCACACG4600.025.29594810-11
ACACGTC4800.023.7475512-13
GCTTGAA4900.022.7773760-61
CAGTCAC4600.022.20106926-27
CTCCAGT3650.019.5204624-25
ACTCCAG5700.019.16411622-23
GTCACAG5850.018.26913328-29
ACAATCT5300.017.92442938-39
CCAGTCA3850.017.88959726-27
GAGCACA8500.017.8802579
TGAACTC4200.017.52772320-21
TCCAGTC6100.016.74172624-25
CTGCTTG7000.016.62370358-59
CACGTCT4450.016.5443914-15
AGCACAC5050.015.9882259
TCTCGTA6550.015.23144942-43
CACACGT5150.015.21687612-13
AACTCCA5150.015.21642222-23
Produced by FastQC (version 0.11.2)