FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l05n01_drought_1h_rep1_root.35100000055c29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l05n01_drought_1h_rep1_root.35100000055c29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19935346
Sequences flagged as poor quality0
Sequence length101
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA4801.8189894E-1210.88490558-59
CTGCTTG5850.010.14915956-57
TTGACCC4100.00553730649.2686472
TGATCTC8350.08.81936294-95
GATCTCG7805.456968E-127.918458594-95
CTTGAAA5153.71625E-77.83871960-61
CCAGTCA18550.07.683679694-95
CTCCAGT22450.07.195392694-95
CAGTCAC17350.06.982848694-95
TCTCGTA7853.2159733E-96.95942340-41
TCCAGTC22550.06.531411694-95
GCCGTCT9101.5079422E-96.524917648-49
CTCGTAT6652.9689163E-66.43002794-95
CACGTCT42650.06.405318394-95
AACTCCA25550.06.321656792-93
ACACGTC45500.06.26515594-95
CACAGTG11604.0017767E-116.14293892-93
GAACTCC27050.06.059597594-95
TGAACTC29950.05.948758694-95
ACTCCAG25700.05.823302794-95
Produced by FastQC (version 0.11.2)