FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l04n02_heat_1h_rep2_shoot.35200000055c33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l04n02_heat_1h_rep2_shoot.35200000055c33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18837625
Sequences flagged as poor quality0
Sequence length101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC353.6046913E-881.3941049
CGGGGTT250.0050365656.959688
AGGGGCG559.493696E-525.90538610-11
AGAGGGG1109.529953E-525.8907648
CGGGAGA950.001228955624.9940644
GGAGAGG1200.00479447719.7714186
TCGGGGG800.001189817.81733938-39
GAGAGGG1350.00945369317.577027
GGGGAAG1451.8577839E-514.74932520-21
GGGCGGG1604.7177662E-513.35996612-13
GGGAAGG1453.0582372E-413.11256822-23
GGCGGGG1902.346908E-411.25049912-13
GGGGGGC2550.00346388978.38606842-43
GGGGCGG2800.0080697197.63283710-11
CGGGGGG3300.00429723927.19842714-15
Produced by FastQC (version 0.11.2)