FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l04n02_heat_1h_rep2_root.35200000055c83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l04n02_heat_1h_rep2_root.35200000055c83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35710786
Sequences flagged as poor quality0
Sequence length101
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG1500.044.3502271
GGTCGGG951.9246727E-840.0152441
ATCGGGA2100.033.9345552
CGGGAGA2400.033.638374
TCGGGGG4450.030.9641638-39
TCTCGGG1400.028.83048236-37
ATGGGAG1004.9512724E-528.5050262
TCGGGAG3000.028.5019073
TTCCGGG1350.028.13955936-37
GAGGGGC1703.6652636E-927.9296939
CCGTGGG350.00838291427.15083938-39
ACGGGAG1056.908409E-527.1446743
GGGGTTC1007.221388E-1026.1218432-33
CTCGGGG1900.024.99236936-37
GGGAGAG4550.023.9978245
GGATGGG1401.7761813E-523.759051
CCGGGTC701.7783646E-523.75533548-49
GGGGGAT1500.023.74799330-31
GAGCGGC1252.264042E-422.7906289
CGGGTTC1053.4455297E-822.6241348-49
Produced by FastQC (version 0.11.2)