FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l04n02_heat_1h_rep1_root.35200000055be1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l04n02_heat_1h_rep1_root.35200000055be1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26102975
Sequences flagged as poor quality0
Sequence length101
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG753.073119E-431.6792131
GAGGGGC953.503314E-529.9879479
TCGGGGG1850.028.250838-39
GAGAGGG1751.501594E-724.4068247
TCCGGGG901.9494473E-723.75635538-39
GGAGAGG1852.571578E-723.0842196
TCTCGGG704.8654544E-420.35109136-37
AGGGGAG857.9424564E-519.56523928-29
TTTCGGG1303.2973003E-718.263836-37
CGGGTTA951.8648626E-417.50345648-49
AGAGGGG1901.878029E-417.4873038
GGGAGAG2202.9352155E-517.263045
CGGGAGA1700.001791482416.7611854
GGCGGGT1157.9806335E-414.45966946-47
GGGGGCC2701.382432E-1014.08021742-43
AGGGGCG1351.6810614E-414.07218410-11
CGGGGAG1250.001498577713.30248614-15
GATGTTC1100.00997382212.944658-59
GGGGGGC5900.012.48426142-43
GAGGGGG3054.7437695E-412.4479257
Produced by FastQC (version 0.11.2)