FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l04n01_heat_1h_rep2_shoot.35100000055c36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l04n01_heat_1h_rep2_shoot.35100000055c36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18837625
Sequences flagged as poor quality0
Sequence length101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA4600.026.8447449
AGAGCAC4500.025.3303578
GTCACCT2950.024.1505728-29
CCTTGTA3150.023.37200732-33
GAACTCC3100.022.9811420-21
CAGTCAC3450.022.0274526-27
ACGCTCT1100.00287187221.6001261
GCTTGAA3550.021.40911958-59
ACTCCAG3400.020.2549222-23
ACACGTC3650.020.16902412-13
AATCTCG3800.019.99985338-39
CTGCTTG3950.019.84286556-57
CCAGTCA2950.019.32068626-27
AGTCACC3000.018.99844728-29
TCCAGTC3500.018.998424-25
AACTCCA3000.018.99771722-23
GCACACG3850.018.50436810-11
TGAACTC3100.018.38491220-21
CTCCAGT3150.018.09371424-25
CTCGTAT3350.017.7257542-43
Produced by FastQC (version 0.11.2)