FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l04n01_heat_1h_rep2_root.35100000055c86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l04n01_heat_1h_rep2_root.35100000055c86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35710786
Sequences flagged as poor quality0
Sequence length101
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG17050.038.0310456-57
ACTCCCG652.055458E-1036.53435522-23
GCTTGAA18150.035.3328458-59
AACTCCC759.586074E-1031.66337620-21
CTCCCGT759.586074E-1031.66310922-23
GCCGTCT21950.030.73014348-49
CGTCTTC21900.030.58415250-51
AATCTCG19700.030.38016538-39
TCTCGTA21400.028.30135340-41
CCCTGAC350.00839650927.1418230-31
GTATGCC25850.027.10555644-45
ATGCCGT27100.025.41709746-47
CTTGAAA19750.025.3711760-61
GACCAAT25000.025.07826834-35
ATCTCGT19500.024.84713640-41
CTCGTAT19700.024.47522742-43
CCAATCT24200.024.14201236-37
GTCTTCT21250.023.1371852-53
AACTCGT1750.023.07101638-39
TCTTCTG29350.023.0640652-53
Produced by FastQC (version 0.11.2)