FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l04n01_heat_1h_rep1_root.35100000055be4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l04n01_heat_1h_rep1_root.35100000055be4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26102975
Sequences flagged as poor quality0
Sequence length101
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG7350.031.99236956-57
GCTTGAA8350.028.44480358-59
GCCGTCT10400.024.89313748-49
CTCGTAT9450.023.6258342-43
CGTCTTC11250.023.01269750-51
TATCTCG11200.022.9012838-39
CTTGAAA9800.022.29401660-61
TCTCGTA11350.022.1811940-41
ATCTCGT11100.020.5410740-41
GTATCTC11200.020.35669338-39
GTCTTCT11400.020.20993852-53
GTATGCC13750.019.00120244-45
TCTGCTT12650.017.4618856-57
CACCGAT14050.017.41024830-31
TGCTTGA13050.017.10829458-59
CCGTCTT14200.016.5592450-51
ATGCCGT16150.016.17752546-47
TGCCGTC14950.015.4105148-49
GTCACCG16200.014.95287228-29
CTTCTGC15600.014.31224154-55
Produced by FastQC (version 0.11.2)