FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l03n02_salt_24h_rep2_root.35200000055c19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l03n02_salt_24h_rep2_root.35200000055c19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42724580
Sequences flagged as poor quality0
Sequence length101
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC857.246854E-944.6958129
TCGGGGG2200.035.6286438-39
GGTCGGG600.003945479631.6782341
CTCGGGG1400.027.13550436-37
GAGAGGG2651.8189894E-1225.0774127
GGAGAGG3050.024.8986286
AGGGGCG1251.4551915E-1124.70316110-11
TCCGGGG1351.8189894E-1224.63214538-39
GATCGGG1000.001651108823.7586751
AGAGGGG3000.023.7344788
CGGGAGA1903.3029573E-722.4927464
GGGAGAG2801.5934347E-920.3444335
AGGGGGC600.004762782719.79419910-11
CGGGTTC757.7241875E-419.00117148-49
GTTCGGG757.7414856E-418.99485236-37
CGGGGAG650.007550485418.27638614-15
TCTCGGG1455.2425094E-818.01236236-37
GGGGGGC5050.017.87523842-43
GGGGCGC1354.872454E-717.59648142-43
GGGAGAT1254.577574E-617.0962930-31