FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l03n01_salt_24h_rep2_shoot.35100000055bbd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l03n01_salt_24h_rep2_shoot.35100000055bbd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28233428
Sequences flagged as poor quality0
Sequence length101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC4500.033.7749758
CCAGTCA3050.026.47340226-27
GAGCACA5950.025.54419
GAACTCC3100.023.74830420-21
CTCCAGT3200.023.00606724-25
ACTCCAG3300.022.30887422-23
CAGTCAC3750.022.16498426-27
GCACACG3750.020.89826810-11
CACGTCT3650.020.82054114-15
TGAACTC3650.020.16979420-21
AACTCCA3550.020.06886522-23
ACACGTC3950.019.84012612-13
CACACGT4050.019.35024612-13
CTGCTTG4200.019.22711656-57
AGCACAC4000.018.99842510-11
TCCAGTC4150.018.8841124-25
CTCGTAT4300.018.78053942-43
ATCTCGT4300.018.22776640-41
GCTTGAA4350.018.01810858-59
CGCCAAT4600.017.5540132-33
Produced by FastQC (version 0.11.2)