FastQCFastQC Report
Thu 19 May 2016
C838DACXX_l03n01_salt_24h_rep2_root.35100000055c1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC838DACXX_l03n01_salt_24h_rep2_root.35100000055c1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42724580
Sequences flagged as poor quality0
Sequence length101
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG11100.027.17485258-59
GCTTGAA11800.024.15118460-61
CTCGTAT13000.020.46335644-45
CGTCTTC14100.020.04377452-53
TCTGCTT15600.019.48820558-59
CTTGAAA14900.019.12534362-63
GATCTCG13850.019.03557440-41
GTCTTCT15050.018.93787454-55
GCCGTCT15050.018.61989850-51
CGATCTC14150.018.12842840-41
TCTCGTA15650.017.30185742-43
ATCTCGT15400.017.27426142-43
TGCTTGA16750.017.0139760-61
TCACAAG1750.002173113616.2838657
GTATGCC20250.015.13097446-47
CTTCTGC21600.013.74493656-57
CCGTCTT21650.013.71209252-53
TGCCGTC24650.011.75370350-51
TCTTCTG27000.011.34781854-55
ATGCCGT25500.011.26951548-49
Produced by FastQC (version 0.11.2)